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| Costa,Mateus Matiuzzi da; Klein,Cátia Silene; Collares,Roberta Mattos; Vaz,Clarissa Silveira Luiz; Balestrim,Raquel; Schrank,Augusto; Silva,Sergio Ceroni da; Piffer,Itamar Antônio; Shrank,Irene Silveira. |
| A pleuropneumonia suína (PPS) provoca prejuízos significativos na suinocultura no mundo. O agente etiológico é a bactéria Actinobacillus pleuropneumoniae (App), que apresenta 15 sorotipos descritos, os quais variam consideravelmente em relação a sua patogenicidade. Nesse sentido, a precisa caracterização patotípica desta bactéria é de grande importância para a adoção de medidas de controle e profilaxia. O diagnóstico e a sorotipificação deste patógeno são realizados pelas técnicas microbiológicas convencionais. Entretanto, problemas nestes esquemas podem ser observados, especialmente em isolados de rebanhos sem histórico de PPS. No Brasil, diversos esforços vêm sendo aplicados no sentido de desenvolver técnicas moleculares que auxiliem no diagnóstico da... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. pleuropneumoniae; Diagnóstico; PCR; RDNA 16S; RAPD. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400057 |
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| Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto. |
| Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009 |
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| Oliveira,Eder Silva de; Junges,Ângela; Sbaraini,Nicolau; Andreis,Fábio Carrer; Thompson,Claudia Elizabeth; Staats,Charley Christian; Schrank,Augusto. |
| Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAGases; GH20 and GH3; Metarhizium; Chitinolytic system; Entomopathogenesis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500843 |
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